2 edition of Cytokine inhibitory actions and gene expression in islets of Langerhans found in the catalog.
Cytokine inhibitory actions and gene expression in islets of Langerhans
Written in English
D.Phil. 2000. BLDSC DXN033012.
|Statement||by Vicky Hadjivassiliou.|
|Series||Sussex theses ; S 4941|
In previous study, we have found that the endonuclease inhibitor aurintricarboxylic acid (ATA) can protect macrophages from cell death induced by bacterial lipopolysaccharide. This action is through the interruption with signalling pathways for NF‐κB and AP‐1 activation, and thus iNOS by: Islets were either infected with V, a CBV-4 strain that has been shown to cause lytic infection in human islets in vitro, or with VD, a CBV-4 strain that causes persistent infection in human islets in vitro. Gene expression in infected islets was compared with that in uninfected control by:
Cytokines also induce COX 2 expression and subsequent prostaglandin production in human islets of Langerhans. Inhibition of COX 2 attenuates cytokine-induced prostaglandin production, but do not. (C) Effect of INH 2 BP on 48 h cytokine‐induced apoptotic cell death in rat islets of Langerhans. Data is expressed as mean± from three separate islet isolations. Statistical analysis was performed using ANOVA where * Pislets and †Pcytokine treated by:
Tsiotra, P., Tsigos, C. & Raptis, S. TNFα and leptin inhibit basal and glucose-stimulated insulin secretion and gene transcription in the HIT-T15 pancreatic cells. Int J O – Cited by: Mouse islets were pretreated with OSA® ( mg/ml) for 8 hr before incubation with OSA® ( mg/ml) in the presence or absence of cytokines (interleukin‐1β + TNF‐α + interferon‐γ) for 16–18 hr. Microarray analysis was performed and the gene expression was assessedAuthor: Altaf Al‐Romaiyan, Bo Liu, Shanta Persaud, Peter Jones.
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Cytokine- or Chemically Derived Nitric Oxide Alters the Expression of Proteins Detected by Two-Dimensional Gel Electrophoresis in Neonatal Rat Islets of Langerhans Nerys E. John, Henrik Ullits Andersen, Stephen J. Fey, Peter Mose Larsen, Peter Roepstorff, Martin R.
Larsen, Flemming Pociot, Allan E. Karlsen, Jørn Nerup, Irene C. Green,Cited by: Cytokine inhibitory actions and gene expression in islets of Langerhans.
Author: Hadjivassiliou, Vicky. Cytokines such as IFN‐γ, IL‐1β and TNF‐α, produced by infiltrating T cells and activated macrophages, are considered key mediators of such inflammatory process and β‐cell death, 2, 3, 18 acting through modification of the expression of complex gene networks as a consequence of the activation of the two transcription factors STAT‐1 Cited by: 1.
Inhibition of inflammatory gene expression by withaferin A. A, Heat map analysis of inflammatory genes demonstrates that compared with control islets, cytokine-treated islets have significant upregulation of RANTES (CCL5), IP10 (CXCL10), MIG (CXCL9), ITAC (CXCL11), TNFα, IL1β, Cited by: Cytokines markedly reduced insulin gene expression and the (pro)insulin biosynthesis rate, which was accompanied by a profound depletion of the islet insulin content.
The cytokines did not affect. Methods: The effects of BMP2 and -4 on beta cell proliferation, apoptosis, gene expression and insulin release were studied in isolated islets of Langerhans from rats, mice and humans.
IL is a homodimeric cytokine with a wide range of inhibitory actions. IL is produced by a variety of cells, including monocytes and T cells, and can exert its effects on Cited by: RNA-seq gene expression results (black bars) were compared to gene expression assessed by qRT-PCR (gray bars) in the 5 human islet preparations used for RNA-seq.
Data were normalized to the geometric mean of β-actin and GAPDH expression and expressed as fold induction of control. *pCited by: The islets of Langerhans comprise approximately % of the weight of the pancreas and are more concentrated in the tail than in the head or body of the pancreas.
Islets can be isolated and separated completely from the exocrine pancreas by the collagenase technique, and the isolated islets will form, store, and release insulin in vitro.
This procedure has made it feasible to accomplish. Global profiling of coxsackievirus-and cytokine-induced gene expression in human pancreatic islets Received: 24 February / Accepted: 29 March / Published online: 1 July In line with studies on the regulation of insulin expression in INS-1 cells or isolated islet cells, treatment with individual cytokines results in a 53–92% inhibition of insulin mRNA levels.
Under these conditions, a significant down-regulation of IA-2 mRNA was observed decreasing to 55–72% compared with basal by: Initial damage, chemokines and checkpoint 1. Although the role of non-lymphoid cells in islet inflammation is not clear, the initial sign of autoimmunity in NOD mice is the presence of macrophages (MØ) and dendritic cells (DC) in the periphery of the islets of Langerhans [1, 2, 3].This infiltration of DC and MØ has been referred to as Checkpoint 1 in the development of diabetes .Cited by: To elucidate the role of cytokine (IL-1beta + gamma-interferon [IFN-gamma])-induced expression of NF-kappaB in beta-cell apoptosis, rat beta-cells were infected with the recombinant adenovirus AdIkappaB((SA)2), which contained a nondegradable mutant form of inhibitory kappaB (IkappaB((SA)2), with S32A and S36A) that locks NF-kappaB in a cytosolic protein complex, Cited by: Notably, both cytokines and viral infection significantly (pexpression of several chemokines, the cytokine IL and the intercellular adhesion molecule ICAM-1, which might contribute to the homing and activation of mononuclear cells in the islets during infection and/or an early autoimmune by: Nitric oxide has been shown to mediate beta-cell destruction in rodent islets exposed to interleukin 1β in culture.
The inhibitory effect is potentiated by tumour necrosis factor-α and interferon- γ. Cytokine stimulation leads to gene transcription and translation of inducible nitric oxide synthase, the biosynthetic enzyme of nitric by: inhibited glucagon gene expression in alphaTC cells.
This inhibitory effect was also observed in isolated mouse islets cultured with leptin, as well as in islets from mice treated with leptin for 5 days. In contrast, no changes were detected in islets from db/db mice, which lack leptin receptors.
Leptin treatment also reduced the glucagonCited by: all, obestatin actions within the endocrine pancreas are still largely unknown. Given that it is encoded by the ghrelin gene, we sought to investigate whether obestatin would display survival effects in pancreatic β-cells and human islets.
In addition, we evaluated whether obestatin would influence the expression of the majorCited by: The immune-mediated elimination of pancreatic beta cells in type 1 diabetes involves release of cytotoxic cytokines such as IL-1β and IFNγ, which induce beta cell death in vitro by mechanisms that are both dependent and independent of nitric oxide (NO).
Nuclear factor kappa B (NFκB) is a critical signalling molecule in inflammation and is required for expression of the gene Cited by: Consistent with the inability to attenuate the inhibitory actions of PGJ2 on cytokine signaling, neither dnPPARgamma nor GW prevents the inhibitory actions of PGJ2 on ILstimulated iNOS gene expression or nitric oxide production by RINm5F by: We report on long-term delivery of an interferon-γ (IFNγ) inhibitory protein by intramuscular (i.m.) gene therapy.
IFNγ is a cytokine that plays an important role in Cited by:. PPARγ inhibition does not attenuate the inhibitory actions of PGJ 2 on cytokine-induced iNOS expression or NO production.
Pancreatic β-cells respond to the proinflammatory cytokine IL-1 by expressing iNOS and producing the free radical NO, and NO is known to mediate β-cell damage stimulated by IL-1 (reviewed in Ref.
15).Cited by: Cytokine-induced expression of BCL2A1 in human islets has been previously observed by array analysis, but the function of this gene in beta cells remained to be clarified. Of interest, BCL2A1 inhibits apoptosis induced by, among others, the BH3 only protein Bim , .Cited by: Glucagon decreases cytokine induction of nitric oxide synthase and action on insulin secretion in RIN5F cells and rat and human islets of Langerhans.
Belin VD(1), Mabley JG, James RF, Swift SM, Clayton HA, Titheradge MA, Green by: